FastQCFastQC Report
Fri 10 Feb 2017
ERR1147972.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147972.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2610176
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138050.5288915383483719No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79870.3059946915457042No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67490.2585649396822283No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA113250.049.163661
GGTATCA65100.046.8338431
AACGCAG139650.039.3126346
TCAACGC144650.037.9948654
ATCAACG146050.037.671383
CAACGCA146950.037.602575
TATCAAC154050.035.9915432
ACGCAGA158300.034.8313457
GTACATG162900.034.106051
CGCAGAG162350.034.0723468
TACATGG165450.033.367792
CATGGGG90050.033.2242854
ACATGGG161500.033.1836133
ATGGGGG49450.031.5142065
GCAGAGT176100.031.4795079
TGGGGGG42000.027.1908846
AGAGTAC170550.026.33095610-11
TATAACG2507.039489E-1026.1757372
CATGGGT28450.025.088254
GAGTACT84900.025.0110512-13
Produced by FastQC (version 0.11.5)