FastQCFastQC Report
Fri 10 Feb 2017
ERR1147971.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147971.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2294460
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112080.48848094976595796No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59760.26045344002510395No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55380.2413639810674407No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24300.10590727229936456No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA79600.055.497011
GGTATCA41900.052.0050851
AACGCAG105350.042.1780136
TCAACGC105850.041.6977964
ATCAACG107700.040.981543
CAACGCA108000.040.9778565
TATCAAC116100.038.1239172
ACGCAGA122700.036.1654857
GTACATG125400.035.560111
CGCAGAG125800.035.321578
TATAACG2250.034.3730162
TACATGG129400.034.159492
ACATGGG127100.034.1178783
ATGGGGG43850.033.6420255
CATGGGG76900.032.797414
GCAGAGT137700.032.3986859
AGAGTAC130050.028.99872810-11
TAACGCA2701.8189894E-1228.6404384
TGGGGGG36150.027.6440096
TATCACG1358.2574756E-526.4407842
Produced by FastQC (version 0.11.5)