FastQCFastQC Report
Fri 10 Feb 2017
ERR1147970.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147970.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2067040
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121290.586781097608174No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64880.3138787831875532No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58230.2817071754779782No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22550.1090931960678071No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA89300.056.9403081
GGTATCA44800.050.1045071
AACGCAG110100.045.8132446
TCAACGC114500.044.156634
ATCAACG115750.043.6283873
CAACGCA116600.043.3103455
TATCAAC123800.040.9405752
ACGCAGA127900.039.530377
CGCAGAG132050.038.242988
GCAGAGT142150.035.6512879
GTACATG132850.035.182081
TACATGG136450.034.0932352
ACATGGG134800.034.0209923
CATGGGG74500.032.814674
ATGGGGG38850.032.1522035
CATGGGT29300.029.2332694
AGAGTAC138800.028.15518610-11
TGGGGGG34400.026.8012926
GAGTACT65550.026.26989612-13
CAGAGTA142100.025.80604710-11
Produced by FastQC (version 0.11.5)