FastQCFastQC Report
Fri 10 Feb 2017
ERR1147969.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147969.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2669772
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148910.5577629850039628No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79030.29601778728670464No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71280.2669890912032938No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA96700.057.320371
GGTATCA48800.052.460741
AACGCAG124250.044.0382046
TCAACGC125550.043.3927274
ATCAACG127400.042.8559843
CAACGCA128950.042.2947245
TATCAAC138400.039.8440252
ACGCAGA145900.037.62577
CGCAGAG149850.036.673598
GTACATG142900.033.8722531
GCAGAGT163950.033.6284269
TACATGG146950.032.8704762
ACATGGG147950.031.9185923
CATGGGG84350.029.6848724
AGAGTAC155650.028.08512710-11
ATGGGGG44650.027.0403775
GAGTACT83650.026.44936212-13
CATGGGT32650.025.5025184
AGTACTT88150.025.47022812-13
CAGAGTA160050.025.34351710-11
Produced by FastQC (version 0.11.5)