FastQCFastQC Report
Fri 10 Feb 2017
ERR1147968.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147968.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1306739
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80380.6151190099935794No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54710.41867580289560496No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37780.2891166483896172No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42250.050.7282831
GTATCAA67250.044.44121
AACGCAG82050.036.896176
TATAGCG650.00194283336.6006435
TCAACGC86450.034.811894
CAACGCA87550.034.442445
ATCAACG87550.034.3065723
TATCAAC91600.032.9883162
ACGCAGA94300.032.2293327
CGCAGAG97200.031.3289458
CGGTATA953.292112E-431.3067782
ACCGTAT800.00537303529.7380228
GTACATG87700.029.5979251
GCAGAGT105500.028.920589
TACATGG89700.028.8462092
ACATGGG89200.028.2711243
TATCACG850.007220863427.9919432
CATGGGG44450.027.6975084
GAGTACT49450.026.8814912-13
ATGGGGG19600.025.7931845
Produced by FastQC (version 0.11.5)