FastQCFastQC Report
Fri 10 Feb 2017
ERR1147967.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147967.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2450381
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134690.5496696228055964No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85280.348027510823827No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65750.26832561956691636No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28570.1165941133236015No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56100.052.3285641
GTATCAA100200.051.226451
AACGCAG127450.040.0467956
TCAACGC129200.039.5504044
CAACGCA131100.039.0679635
ATCAACG131750.038.7849123
TATCAAC147250.034.827752
ACGCAGA148100.034.462967
CGCAGAG152450.033.440578
GTACATG141950.032.258611
GCAGAGT163000.031.3491549
TACATGG146500.031.1480772
ACATGGG144450.030.762623
CATGGGG76650.030.1896274
CATGGGT27200.029.0872754
AGAGTAC155100.027.48050510-11
GAGTACT77550.025.73540712-13
CAGAGTA160200.025.06464410-11
AGTACTT84700.024.6164212-13
ATGGGGG38850.024.3459325
Produced by FastQC (version 0.11.5)