FastQCFastQC Report
Fri 10 Feb 2017
ERR1147966.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147966.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2619892
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154080.5881158459967052No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104400.3984897087360853No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82520.31497481575576397No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31810.12141721872504668No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31420.11992860774413601No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT27080.10336303939246351No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA132000.050.662341
GGTATCA75650.049.8280831
AACGCAG157700.042.171286
TATAACG2200.040.56642
TCAACGC169100.039.222744
ATCAACG171150.038.752943
CAACGCA172800.038.4861765
ACGCAGA185150.035.9190447
TATCAAC185950.035.8360142
CGCAGAG188150.035.251488
GTACATG187100.033.769221
TACATGG193050.032.7613142
ACATGGG190750.032.5568733
GCAGAGT204950.032.4489449
CATGGGG102800.031.0733364
ATGGGGG49200.031.0723935
CATGGGT41050.029.9960384
AGAGTAC197150.028.52800810-11
TGGGGGG39650.028.2046936
CAGAGTA200750.025.912610-11
Produced by FastQC (version 0.11.5)