FastQCFastQC Report
Fri 10 Feb 2017
ERR1147965.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147965.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2530123
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137610.5438866015604775No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75260.2974558944367527No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69130.27322782331135675No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA101550.055.347731
GGTATCA54600.046.6722681
AACGCAG126800.044.232876
TCAACGC130850.042.7274324
CAACGCA133750.042.023355
ATCAACG133800.041.6964763
TATCAAC140650.040.0941772
ACGCAGA147150.038.1965527
CGCAGAG150700.037.3757068
GCAGAGT163800.034.386569
GTACATG150000.033.5804751
TACATGG154500.032.572762
ACATGGG152650.032.378513
TATCACG1951.4260877E-930.5054972
CATGGGG83550.029.0446644
CATGGGT31950.028.8544964
AGAGTAC157300.028.5665910-11
AGTACTT82700.025.9270112-13
GAGTACT79450.025.86466212-13
CAGAGTA162200.025.24676510-11
Produced by FastQC (version 0.11.5)