FastQCFastQC Report
Fri 10 Feb 2017
ERR1147964.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147964.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1969859
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79790.40505437191189825No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46800.23758045626615915No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38510.19549622587200405No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA68350.052.0087131
GGTATCA37150.043.916941
AACGCAG84900.041.5444536
TCAACGC85950.040.8985254
ATCAACG87450.040.128993
CAACGCA87750.040.059585
TATCAAC94300.037.5331842
ACGCAGA97950.036.0701567
CGCAGAG99850.035.4433638
GTACATG99250.033.1168371
GCAGAGT110250.032.0999539
TACATGG102300.031.9813792
ACATGGG101700.030.9970933
GCGTATC1254.8596263E-528.5812721
CATGGGG57250.027.84364
AGAGTAC104300.026.57465710-11
ATGGGGG29400.026.3003835
CATGGGT22750.025.3604164
GAGTACT50700.024.63640812-13
CAGAGTA107350.024.21282210-11
Produced by FastQC (version 0.11.5)