FastQCFastQC Report
Fri 10 Feb 2017
ERR1147963.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147963.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2155926
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115910.5376344086021506No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66410.30803469135768113No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57070.26471223965943175No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25480.11818587465432487No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA82700.058.6656951
GGTATCA42300.054.463141
AACGCAG109500.044.3758856
TCAACGC110100.044.0260164
ATCAACG111400.043.458863
CAACGCA113150.042.9444055
TATCAAC118350.041.117332
ACGCAGA128950.037.9592447
CGCAGAG133600.036.593548
GCAGAGT142700.034.3016479
GTACATG132500.034.010431
ACATGGG133800.033.293913
TACATGG136300.032.9526942
CATGGGG79350.031.7803024
ATGGGGG46300.031.7289035
TGGGGGG38900.028.1324376
AGAGTAC137000.028.04471810-11
CATGGGT26000.027.4502944
GAGTACT66950.026.2065912-13
CAGAGTA140750.025.56501410-11
Produced by FastQC (version 0.11.5)