FastQCFastQC Report
Fri 10 Feb 2017
ERR1147962.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147962.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2804457
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154370.5504452377055523No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87360.3115041521406818No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72950.26012165634916135No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA124500.048.4928251
GGTATCA73050.041.0789381
AACGCAG148900.040.3810166
TCAACGC158450.037.9847344
ATCAACG160200.037.6811683
CAACGCA160900.037.443315
TATCAAC175000.034.8732222
ACGCAGA172850.034.8546647
CGCAGAG177850.034.0085338
GTACATG178600.032.10361
GCAGAGT191700.031.6135229
TACATGG182650.031.2632622
ACATGGG179550.030.8710423
CATGGGG102500.028.4890824
CATGGGT34500.028.0989024
AGAGTAC183200.026.5389110-11
ATGGGGG44950.025.8003585
GAGTACT84250.025.3422612-13
AGTACTT88450.025.18110512-13
CAGAGTA187350.024.30033710-11
Produced by FastQC (version 0.11.5)