FastQCFastQC Report
Fri 10 Feb 2017
ERR1147961.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147961.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3108948
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172390.5544962476052993No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102690.3303046561087545No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80100.2576434215046376No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79100.048.015811
GTATCAA132600.046.870221
AACGCAG162200.038.0989846
TCAACGC170750.036.1564144
ATCAACG171550.035.8843733
CAACGCA173500.035.617615
TATCAAC180200.034.3615382
ACGCAGA188950.032.642297
CGCAGAG194250.031.8128978
GTACATG180800.031.7408561
TACATGG188000.030.689552
ACATGGG183750.030.556273
GCAGAGT209550.029.6888089
CATGGGG99650.028.947644
CATGGGT39300.026.938674
AGAGTAC200600.025.27622410-11
GAGTACT102400.025.00471512-13
CAGAGTA206150.024.35049610-11
AGTACTT106400.023.50569312-13
ATGGGGG49100.023.3789275
Produced by FastQC (version 0.11.5)