FastQCFastQC Report
Fri 10 Feb 2017
ERR1147960.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147960.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3316957
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201090.6062484379508085No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109950.331478520824961No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97580.2941853029749858No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA140900.055.135961
GGTATCA74650.050.0003621
AACGCAG178400.043.1074836
TCAACGC186350.041.3322754
ATCAACG187100.041.1036343
CAACGCA189150.040.6575435
TATCAAC200200.038.7746432
ACGCAGA208200.037.0231447
CGCAGAG214750.035.921618
GCAGAGT231650.033.377989
GTACATG202750.031.211011
ACATGGG206400.030.3440953
TACATGG208050.030.335352
CATGGGG110250.029.6710594
AGAGTAC222050.028.2050310-11
ATGGGGG50850.025.7324245
GAGTACT112750.025.58433712-13
AGTACTT118600.025.22506312-13
CAGAGTA228950.025.0689210-11
CATGGGT40950.023.2388734
Produced by FastQC (version 0.11.5)