FastQCFastQC Report
Fri 10 Feb 2017
ERR1147958.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147958.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3552356
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172850.486578484814022No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95790.2696520281188034No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84580.23809550619363598No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA134950.053.2136571
GGTATCA73900.044.397231
AACGCAG169600.042.084976
TCAACGC178000.040.065524
ATCAACG180250.039.53243
CAACGCA181100.039.2811665
TATCAAC194350.036.7628822
ACGCAGA196750.036.3984877
CGCAGAG200550.035.6791538
GTACATG196350.034.268591
TACATGG202500.032.9917872
GCAGAGT218600.032.8147249
ACATGGG201500.032.6771553
CATGGGG115900.031.048714
ATGGGGG56800.029.7400465
CATGGGT44350.028.4324234
AGAGTAC208550.028.39243910-11
TGGGGGG44900.026.6269456
AGTACTT115200.025.42877212-13
GAGTACT107850.024.65238812-13
Produced by FastQC (version 0.11.5)