FastQCFastQC Report
Fri 10 Feb 2017
ERR1147957.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147957.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2894567
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT208660.7208677498223396No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108600.37518564952892786No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102270.3533170937138439No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30880.10668262299680746No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA168300.048.2663961
AACGCAG196700.041.067626
TCAACGC216350.037.2826654
ATCAACG217150.037.1453133
TATCAAC220950.036.7313652
GGTATCA101700.036.716491
CAACGCA220300.036.668195
ACGCAGA222200.036.4884957
CGCAGAG224500.036.1411678
GCAGAGT244800.033.2656679
GTACATG221350.033.2009281
TACATGG225500.032.560072
ACATGGG218950.032.5473823
CATGGGG112550.032.3452154
CATGGGT42100.030.8019774
ATGGGGG52050.029.5994195
AGAGTAC236350.027.20659810-11
GAGTACT115500.026.162912-13
AGTACTT122650.025.97144512-13
CAGAGTA242850.024.49435810-11
Produced by FastQC (version 0.11.5)