FastQCFastQC Report
Fri 10 Feb 2017
ERR1147956.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147956.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3667443
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187150.5103010462602964No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108520.29590098605486165No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87130.2375769712030971No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA145500.051.562731
GGTATCA79150.049.5750161
AACGCAG176550.042.245876
TCAACGC187450.039.9479684
ATCAACG189650.039.453743
CAACGCA191600.039.113755
TATCAAC201850.037.309942
ACGCAGA204050.036.6106537
CGCAGAG209500.035.8002058
GCAGAGT227600.033.0054369
GTACATG204050.032.4194641
TACATGG212200.031.0608942
ACATGGG209400.030.7334233
CATGGGG120150.028.9096954
AGAGTAC216750.027.56419210-11
CATGGGT41550.027.1980044
ATGGGGG58450.026.2536455
GAGTACT107150.025.61733612-13
CAGAGTA222900.025.5895710-11
AGTACTT116650.023.8624812-13
Produced by FastQC (version 0.11.5)