FastQCFastQC Report
Fri 10 Feb 2017
ERR1147955.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147955.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3354424
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143990.4292540239397286No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85620.25524501374900727No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68380.20385019902075585No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61050.053.732571
GTATCAA112400.052.755131
AACGCAG141750.041.328626
TCAACGC144300.040.6394964
CAACGCA146500.039.9886135
ATCAACG148600.039.5041353
TATCAAC167250.035.280532
ACGCAGA167850.034.8667537
CGCAGAG173200.033.8584258
GTACATG174800.031.5044561
GCAGAGT188800.031.0923049
TACATGG178100.030.9269162
ACATGGG176600.030.2770253
CATGGGT42650.027.890014
CATGGGG99450.026.1943174
AGAGTAC178550.025.98199510-11
GAGTACT85650.025.13731812-13
CAGAGTA184150.024.93350410-11
ATGGGGG47450.023.4392195
AGTACTT91800.022.18991512-13
Produced by FastQC (version 0.11.5)