FastQCFastQC Report
Fri 10 Feb 2017
ERR1147954.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147954.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3065357
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162660.5306396612205365No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94090.306946303481128No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77440.25262962845763154No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31620.10315274860318063No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA115300.055.9774631
GGTATCA61300.053.3242871
AACGCAG151300.042.457916
TCAACGC153150.041.8285184
ATCAACG155400.041.1463433
CAACGCA157150.040.839545
TATCAAC169550.038.067582
ACGCAGA178300.036.061867
CGCAGAG182550.035.2222948
GCAGAGT196800.032.853249
GTACATG182450.032.1117821
ACATGGG184550.031.2630583
TACATGG187000.031.238722
ATGGGGG62750.030.1430455
CATGGGG110150.030.077694
AGAGTAC191100.026.92335510-11
CAGAGTA194600.025.17065610-11
GAGTACT88500.025.06913412-13
ATATGTA19750.024.7206541
AGTACTT93550.024.51062412-13
Produced by FastQC (version 0.11.5)