FastQCFastQC Report
Fri 10 Feb 2017
ERR1147952.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147952.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2193032
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135680.6186868226273032No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83540.38093379394372723No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66380.30268596171875284No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29110.13273860116952238No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25860.11791893597539845No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA97700.053.865381
GGTATCA53000.053.2420161
AACGCAG124450.042.2964676
TCAACGC127400.041.2703934
ATCAACG128600.040.8399663
CAACGCA129650.040.4624185
TATCAAC132750.039.7533382
ACGCAGA148150.035.4498837
CGCAGAG151450.034.638188
GTACATG147800.033.5120431
GCAGAGT160900.032.677749
TACATGG150950.032.6348572
ACATGGG151350.031.9895153
CATGGGG85100.031.2416324
ATGGGGG45500.029.0199975
GTATCGC2006.143273E-826.7650646
CATGGGT30500.025.7412624
AGAGTAC158450.025.22509410-11
CAGAGTA161050.025.11330810-11
GAGTACT78700.025.09106612-13
Produced by FastQC (version 0.11.5)