FastQCFastQC Report
Fri 10 Feb 2017
ERR1147950.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147950.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2432276
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129710.5332865184707656No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75300.309586576523388No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61660.2535074144546096No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA105050.050.6099971
GGTATCA60000.043.560631
AACGCAG126650.041.3769656
TCAACGC134700.038.9041754
CAACGCA135650.038.5878685
ATCAACG136250.038.5052533
TATCAAC143800.036.7769242
ACGCAGA149500.035.0925837
CGCAGAG152100.034.4927068
GCAGAGT165700.031.9488629
GTACATG146000.030.9098761
ACATGGG146300.030.0460953
TACATGG150450.029.9715772
CATGGGG77550.028.7632124
AGAGTAC159150.026.83525510-11
ATGGGGG38400.026.4882225
CATGGGT29800.026.3478744
TATAACG2057.791459E-826.1168752
GAGTACT78350.025.28093512-13
AGTACTT82500.024.62207612-13
Produced by FastQC (version 0.11.5)