FastQCFastQC Report
Fri 10 Feb 2017
ERR1147949.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147949.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3481164
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150210.4314936038635353No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89440.25692555708378No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73160.21015959029795783No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA117400.051.5819661
GGTATCA64300.048.9880031
AACGCAG142250.041.8166926
TCAACGC148100.040.1247524
CAACGCA151200.039.380775
ATCAACG151750.039.198843
TATCAAC166200.036.261252
ACGCAGA165550.035.9672167
CGCAGAG171000.034.7165348
GTACATG172400.034.5388451
TACATGG177150.033.4825362
ACATGGG175250.033.3654823
GCAGAGT185450.032.1718529
CATGGGG98750.030.7209784
AGAGTAC175850.027.06135610-11
ATGGGGG46450.026.2524645
GAGTACT85450.025.40871812-13
CATGGGT38900.025.3840224
CAGAGTA182800.024.9098410-11
AGTACTT92350.023.76793512-13
Produced by FastQC (version 0.11.5)