FastQCFastQC Report
Fri 10 Feb 2017
ERR1147948.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147948.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2546515
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT241440.9481192924447726No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134390.5277408536764951No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122680.4817564396832534No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39240.15409294663491085No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31570.12397335181610945No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA180350.045.8840031
GGTATCA109650.040.9921721
AACGCAG214700.038.4782036
TATCAAC231000.035.949662
TCAACGC233750.035.4186674
ATCAACG233850.035.327913
CAACGCA239100.034.5764055
GTACATG231750.033.523811
ATGGGGG62250.033.440545
ACGCAGA248800.033.276187
CGCAGAG251900.032.913898
ACATGGG231800.032.8691753
TACATGG237650.032.740952
CATGGGG127900.032.3655744
GCAGAGT272050.030.5853589
CATGGGT32950.029.7833214
TATCACG1852.8785507E-828.9395732
TGGGGGG52900.028.4452296
AGAGTAC262850.026.03294210-11
GAGTACT136350.025.84507812-13
Produced by FastQC (version 0.11.5)