FastQCFastQC Report
Fri 10 Feb 2017
ERR1147947.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147947.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3264879
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191390.5862085547427638No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106610.32653583792845003No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91520.2803166671720453No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33290.10196396252357286No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA144550.053.504911
GGTATCA80400.046.1354261
AACGCAG177350.043.4647456
TCAACGC189350.040.6473584
ATCAACG189300.040.5650753
CAACGCA192000.040.0243845
TATCAAC202900.038.1151662
GTACATG219550.037.939081
ACGCAGA208250.037.1297237
TACATGG224250.036.873882
ACATGGG222800.036.307843
CGCAGAG213200.036.211868
CATGGGG126600.035.0484244
ATGGGGG64850.034.761035
GCAGAGT227750.033.9767849
CATGGGT42500.029.3896034
TGGGGGG56000.028.1462926
AGAGTAC220900.027.57195910-11
TATACCG3050.027.3018175
GAGTACT111000.025.98855212-13
Produced by FastQC (version 0.11.5)