FastQCFastQC Report
Fri 10 Feb 2017
ERR1147946.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147946.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4358146
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195620.4488605934725455No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111160.25506258854109065No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94570.2169959427701596No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA156900.051.2962881
GGTATCA88850.047.3019941
AACGCAG197150.040.7555666
TCAACGC203650.039.3379364
ATCAACG207700.038.628593
CAACGCA209050.038.293345
TATCAAC223950.036.0414282
ACGCAGA233050.034.630537
GTACATG235450.034.410551
CGCAGAG239550.033.7156838
TACATGG244600.033.3148082
ACATGGG243850.032.828853
GCAGAGT259150.031.1198049
CATGGGG132000.030.7283214
CATGGGT58850.027.6906074
ATGGGGG67800.027.5440855
AGAGTAC247150.026.41023810-11
GAGTACT125900.024.77696812-13
AGTACTT133250.024.21368412-13
CAGAGTA254250.024.1288510-11
Produced by FastQC (version 0.11.5)