FastQCFastQC Report
Fri 10 Feb 2017
ERR1147945.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147945.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2864830
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174970.6107517723564748No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106670.3723432105919025No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87810.3065103339465169No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33540.11707500968643865No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31140.10869754924375966No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA115950.055.302771
GGTATCA62750.053.703751
AACGCAG151650.042.0864376
TCAACGC152650.041.6938324
ATCAACG154950.041.0749473
CAACGCA156100.040.810455
TATCAAC165700.038.8483852
ACGCAGA178650.035.7257657
CGCAGAG181700.035.0933388
GCAGAGT194550.032.8671539
GTACATG168550.030.4141961
ACATGGG167600.029.847423
TACATGG170500.029.8338972
CATGGGG94500.028.5764884
ATGGGGG54700.027.511745
AGAGTAC189150.027.50035510-11
GAGTACT94400.025.70827912-13
AGTACTT98500.025.66478212-13
CAGAGTA192950.025.40196210-11
CATGGGT38050.023.9178224
Produced by FastQC (version 0.11.5)