FastQCFastQC Report
Fri 10 Feb 2017
ERR1147944.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147944.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2265082
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153720.6786509274277929No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96940.42797567593579394No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77090.3403408794913385No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26770.11818556679184242No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26440.11672866589377338No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA117250.052.454491
GGTATCA64300.051.575021
AACGCAG146100.041.5651636
TCAACGC154150.039.394554
ATCAACG156200.038.9156073
CAACGCA157300.038.605665
TATCAAC160450.038.111432
ACGCAGA172400.035.50037
TATCACG851.7136737E-434.9907262
CGCAGAG176550.034.632148
GCAGAGT187450.032.650059
GTACATG156600.031.4799291
ACATGGG158350.030.837493
TACATGG160800.030.7039282
CATGGGG87850.030.5343534
ATGGGGG44500.028.335435
AGAGTAC180000.027.73972510-11
CATGGGT29900.026.8544654
AGTACTT98450.025.91764612-13
CAGAGTA184600.025.58248710-11
Produced by FastQC (version 0.11.5)