FastQCFastQC Report
Fri 10 Feb 2017
ERR1147943.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147943.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5004035
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT258790.5171626497416585No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143670.28710830359899564No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128710.25721242956933754No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA188950.055.2742541
GGTATCA99900.047.027481
AACGCAG238500.043.443696
TCAACGC246350.042.083494
ATCAACG249850.041.6133
CAACGCA251400.041.26185
TATCAAC270150.038.6251072
ACGCAGA277400.037.415887
CGCAGAG283850.036.5656668
GCAGAGT310550.033.4601869
GTACATG278700.033.0303151
TACATGG284700.032.0540542
ACATGGG279600.031.9733963
CATGGGG158250.029.5799874
ATGGGGG85050.029.3725685
AGAGTAC298100.028.27319110-11
CATGGGT63400.026.2685284
TATAACG4250.025.195832
AGTACTT164800.025.01168812-13
GAGTACT153700.024.70893312-13
Produced by FastQC (version 0.11.5)