FastQCFastQC Report
Fri 10 Feb 2017
ERR1147942.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147942.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2166848
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165640.7644283309212275No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88000.4061198570458103No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77800.3590468736155005No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25330.11689790885193607No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA119900.049.207661
GGTATCA68400.043.3462451
AACGCAG138700.042.1087236
ATCAACG153850.038.0394863
TCAACGC154250.037.9408454
TATCAAC156550.037.6148452
CAACGCA156450.037.36935
ACGCAGA160300.036.3975837
CGCAGAG163600.035.5906948
GCAGAGT177800.032.7816939
GTACATG144500.031.354151
ACATGGG143500.030.5873263
TACATGG147250.030.3766352
CATGGGG70050.030.3107814
AGAGTAC171050.027.6427410-11
CATGGGT29500.026.4110894
GAGTACT93200.026.19595712-13
ATGGGGG26250.025.8292985
AGTACTT97400.025.4327412-13
CAGAGTA176250.025.00495710-11
Produced by FastQC (version 0.11.5)