FastQCFastQC Report
Fri 10 Feb 2017
ERR1147941.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147941.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4105857
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT288610.7029226785053644No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165540.40318013998052055No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140780.3428760426873123No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA219150.049.3109661
AACGCAG237800.045.1442876
GGTATCA124900.040.185881
ACGCAGA274850.039.1886377
CGCAGAG278600.038.6824958
TCAACGC278850.038.6264884
ATCAACG280250.038.454753
CAACGCA282150.038.1747175
TATCAAC291150.037.2652172
GCAGAGT301600.035.7720159
GTACATG250050.035.5739481
ACATGGG250100.034.8386573
TACATGG258700.034.259852
CATGGGG137100.032.969594
CATGGGT50850.030.4102384
ATGGGGG61900.029.8818285
AGAGTAC293800.029.18100510-11
GAGTACT165400.026.96889712-13
CAGAGTA302250.026.23017910-11
AGTACTT182200.025.93480312-13
Produced by FastQC (version 0.11.5)