FastQCFastQC Report
Fri 10 Feb 2017
ERR1147940.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147940.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4597351
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT255690.5561681063725611No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140390.30537150633049337No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129480.2816404490324972No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA206000.049.1012761
AACGCAG235900.042.537876
GGTATCA117000.040.8598061
TCAACGC259150.038.675624
ATCAACG263550.038.0299263
CAACGCA263900.038.0245675
ACGCAGA268650.037.551537
CGCAGAG273800.036.7800338
TATCAAC278700.036.351162
GTACATG250900.034.7619061
GCAGAGT296450.034.0902759
ACATGGG254250.033.45523
TACATGG261650.033.1722642
TATAACG4750.032.562692
CATGGGG136200.031.968524
CATGGGT52500.030.0244564
AGAGTAC287250.028.555710-11
ATGGGGG66450.026.9438765
TAACGCA5300.026.9354324
GAGTACT161700.026.4488912-13
Produced by FastQC (version 0.11.5)