FastQCFastQC Report
Fri 10 Feb 2017
ERR1147939.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147939.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3993995
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210570.527216483746224No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118030.2955186473693633No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106390.26637489531158653No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40860.10230358325436062No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA156250.052.5864451
GGTATCA87800.046.9951971
AACGCAG197600.041.3588526
TCAACGC206500.039.4899024
ATCAACG207850.039.2339063
CAACGCA210250.038.9270175
TATCAAC223900.036.718752
ACGCAGA230750.035.5202647
CGCAGAG236550.034.5236138
GTACATG227650.032.2747041
GCAGAGT256950.031.8984269
TACATGG234500.031.35522
ACATGGG235550.030.75663
CATGGGG132650.029.6389544
AGAGTAC244500.027.42877410-11
ATGGGGG70350.026.8863415
AGTACTT131300.025.07388312-13
GAGTACT124600.024.63203812-13
CAGAGTA252550.024.5408210-11
CATGGGT52600.024.085884
Produced by FastQC (version 0.11.5)