FastQCFastQC Report
Fri 10 Feb 2017
ERR1147938.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147938.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2956374
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT229100.7749357828204415No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125260.4236947016852401No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110910.37515551144746906No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35480.12001187941715087No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32260.10912015868087055No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA155350.055.7644271
GGTATCA81450.049.6345631
AACGCAG200050.043.2334446
ATCAACG205800.042.026933
TATCAAC206550.041.96572
TCAACGC205950.041.9082574
CAACGCA210900.040.9810455
ACGCAGA234550.037.0010347
CGCAGAG237900.036.4800038
GCAGAGT252100.034.5667659
GTACATG219050.033.0518531
TACATGG222450.032.547552
ACATGGG219000.032.295833
TATAACG2950.032.266962
ATGGGGG58050.030.8431175
CATGGGG111350.030.3425524
CATGGGT44750.028.9772684
TAACGCA3550.028.484894
AGAGTAC249650.027.6746210-11
GAGTACT132000.025.8886912-13
Produced by FastQC (version 0.11.5)