FastQCFastQC Report
Fri 10 Feb 2017
ERR1147937.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147937.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2064110
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142070.6882869614506979No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82260.39852527239342866No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66030.3198957419904947No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22790.11041078237109456No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA115600.049.0402151
GGTATCA66050.045.0785981
AACGCAG139650.040.291946
TCAACGC148500.037.89074
ATCAACG150000.037.4730533
CAACGCA150900.037.2880675
TATCAAC151850.037.1804052
ACGCAGA161650.034.918737
CGCAGAG165550.034.0961238
TATCACG1754.474714E-1033.9957472
GCAGAGT179600.031.4288049
GTACATG149950.030.380031
ACATGGG149500.029.6012613
TACATGG153600.029.552622
CATGGGG83600.028.8148734
CGTATAC1107.7677943E-427.0368333
ATGGGGG42250.026.4666585
TATAACG2057.785093E-826.1186872
AGAGTAC175250.026.04883810-11
GAGTACT90300.025.12897512-13
Produced by FastQC (version 0.11.5)