FastQCFastQC Report
Fri 10 Feb 2017
ERR1147936.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147936.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4062970
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT228000.5611658466589712No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125470.3088135034223733No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105760.26030219273093325No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA161950.056.7039871
GGTATCA85800.050.8776251
AACGCAG210200.043.181986
TCAACGC215250.042.224154
ATCAACG218400.041.5611883
CAACGCA218650.041.540365
TATCAAC229350.039.7119562
ACGCAGA246850.036.8671047
CGCAGAG252500.036.0657128
GCAGAGT271700.033.5827649
GTACATG238600.033.4210661
TACATGG247800.032.0977752
ACATGGG245750.031.8044513
CATGGGG141600.029.6986624
ATGGGGG74600.028.704135
AGAGTAC260750.028.0583110-11
CATGGGT49800.027.3518584
GTAAGTA27900.026.8665072
GAGTACT130100.026.24310912-13
AGTACTT138100.025.73504812-13
Produced by FastQC (version 0.11.5)