FastQCFastQC Report
Fri 10 Feb 2017
ERR1147935.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147935.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3354580
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT216140.6443131479946819No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123930.36943521990830447No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107960.3218286640950581No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34760.10361952912138032No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA162650.050.551571
GGTATCA92850.045.07841
AACGCAG195350.041.713326
TCAACGC211300.038.508294
ATCAACG213300.038.2029883
CAACGCA215000.037.873255
TATCAAC225200.036.4532782
ACGCAGA229050.035.576067
CGCAGAG233850.034.7695248
GTACATG216700.032.612711
GCAGAGT255000.032.0023279
ACATGGG219550.031.6429543
TACATGG222450.031.6357562
CATGGGG120000.030.8797974
AGAGTAC243750.027.500910-11
CATGGGT43000.026.0050094
ATGGGGG59000.025.8080755
AGTACTT131500.025.7593912-13
GAGTACT125350.025.38616412-13
CAGAGTA250300.024.61878210-11
Produced by FastQC (version 0.11.5)