FastQCFastQC Report
Fri 10 Feb 2017
ERR1147934.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147934.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3549263
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154740.4359778353985038No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77250.21765081933911348No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73610.20739516908158118No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA120350.056.448271
AACGCAG149850.044.6552246
GGTATCA60950.044.545331
TCAACGC154500.043.4267274
CAACGCA156850.042.7002455
ATCAACG157500.042.5995523
TATCAAC169950.039.8355672
ACGCAGA174050.038.4805157
CGCAGAG181750.036.8829778
GTACATG187050.035.1098441
GCAGAGT198100.034.1091049
TACATGG192450.034.0344922
ACATGGG190500.033.440363
CATGGGG112050.030.4702844
AGAGTAC187550.028.36872110-11
ATGGGGG59300.027.9850435
CATGGGT40550.026.6966654
AGTACTT98500.024.69810312-13
GAGTACT92450.024.54506112-13
CAGAGTA196350.024.12855710-11
Produced by FastQC (version 0.11.5)