FastQCFastQC Report
Fri 10 Feb 2017
ERR1147933.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147933.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3062648
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141400.4616919737429832No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86250.28161904338990307No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67020.21883024102018908No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA115000.052.042961
GGTATCA63900.050.511731
AACGCAG139700.042.8782776
TCAACGC148150.040.1515774
CAACGCA149800.039.907875
ATCAACG151400.039.2903143
TATCAAC161150.037.290192
ACGCAGA166250.036.137997
CGCAGAG170450.035.177738
GTACATG168900.032.014741
GCAGAGT188400.031.8577129
ACATGGG169350.031.2268313
TACATGG174400.030.8749082
CATGGGG96450.029.233474
ATGGGGG49500.027.9998055
CATGGGT34750.026.5326824
GAGTACT85550.026.31783512-13
AGAGTAC179350.026.20173310-11
CAGAGTA185650.024.86410-11
AGTACTT92950.023.90261812-13
Produced by FastQC (version 0.11.5)