FastQCFastQC Report
Fri 10 Feb 2017
ERR1147932.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147932.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1695411
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209771.237281107648824No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120940.7133373559567562No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103310.6093507709929923No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35160.2073833424461679No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22470.13253423506158685No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA19400.11442653138383554No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA157350.047.6725851
AACGCAG177950.042.1772046
GGTATCA92550.041.10451
TATCAAC201750.037.26832
ATCAACG203600.036.8344083
TCAACGC203600.036.80524
ACGCAGA205800.036.5562447
CAACGCA206600.036.328335
CGCAGAG209300.035.916528
GCAGAGT221850.034.0455789
TATAACG1352.3360553E-630.8439752
GTACATG178200.030.7024291
ACATGGG177450.030.2641283
TACATGG180650.030.0963822
CATGGGG95000.029.4858534
CATGGGT25500.029.15324
AGAGTAC220400.027.84739110-11
ATGGGAG18200.027.1220875
ATGGGTG19150.026.708295
ATGGGGT52150.026.685665
Produced by FastQC (version 0.11.5)