FastQCFastQC Report
Fri 10 Feb 2017
ERR1147931.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147931.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2641202
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174590.6610247909853165No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101620.38474906500903755No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83200.3150080910131069No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32500.12305003555199488No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30000.11358464820184144No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA120950.054.9809651
GGTATCA63700.052.7114221
AACGCAG157150.042.0117386
TCAACGC158150.041.746094
ATCAACG159750.041.2907453
CAACGCA161650.040.7686275
TATCAAC165400.039.993452
ACGCAGA184500.035.9129337
CGCAGAG189600.034.9155468
GCAGAGT202750.032.6803259
GTACATG179750.031.5637861
ACATGGG180150.030.9032143
TACATGG184850.030.7650782
CATGGGG99550.029.3958444
ATGGGGG56100.027.7779675
TATAACG3000.027.7604432
GGGTTAG7950.027.7076471
GAGTACT94900.026.91900412-13
AGAGTAC196350.026.83891110-11
AGTACTT100050.026.33593212-13
Produced by FastQC (version 0.11.5)