FastQCFastQC Report
Fri 10 Feb 2017
ERR1147929.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147929.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1243914
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136821.099915267454181No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95790.7700693134734394No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69910.5620163451814193No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24280.1951903427407361No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14200.11415580176764631No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA12860.1033833528684459No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67050.048.389471
GTATCAA110250.044.3860361
AACGCAG123150.039.9944536
TCAACGC142200.034.5528834
ATCAACG142700.034.390133
ACGCAGA145150.034.1784867
CAACGCA144650.034.008775
CGCAGAG147600.033.6111648
GTACATG132900.033.5065541
ATGGGGG32500.033.311325
TATCAAC147750.033.2576332
CATGGGT20050.032.9315724
TACATGG134800.032.833852
ACATGGG135600.032.374313
CATGGGG75100.031.6828274
GCAGAGT157850.031.4286219
AGTACAC16900.028.8623033
TGGGGGG25800.027.6672126
AGAGTAC155450.026.80533810-11
ATGGGAG15450.026.5658955
Produced by FastQC (version 0.11.5)