FastQCFastQC Report
Fri 10 Feb 2017
ERR1147927.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147927.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1827130
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169570.9280675157213771No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101740.5568295633041984No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86680.4744052147356783No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27380.14985250091673827No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22160.12128310519776918No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA119550.053.1325341
GGTATCA66000.047.083881
AACGCAG142850.044.34646
ATCAACG157450.040.2720343
TCAACGC158850.039.8796584
TATCAAC160800.039.6952322
CAACGCA161800.039.1893165
ACGCAGA164400.038.424817
CGCAGAG167400.037.6651278
GCAGAGT179150.035.1947639
ATGGGGG41850.034.8224755
GTACATG149400.034.108991
ACATGGG151550.033.401243
TACATGG154150.033.0335242
TATAACG1451.1004704E-732.820612
CATGGGG87500.032.6983644
TGGGGGG33600.031.8656126
AGAGTAC173500.028.8841410-11
CATGGGT24750.028.359324
TATCACG1505.305381E-627.7607672
Produced by FastQC (version 0.11.5)