FastQCFastQC Report
Fri 10 Feb 2017
ERR1147926.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147926.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1824143
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113850.6241287004363145No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62420.342188085035No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54880.30085360632362707No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24280.13310359988224607No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC18820.10317173598780359No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA83400.055.978311
GGTATCA43950.046.608951
AACGCAG103850.044.9021536
TCAACGC110300.042.2224924
ATCAACG111700.041.5867923
CAACGCA112250.041.541995
TATCAAC117650.039.6912542
ACGCAGA123150.037.9134037
CGCAGAG125350.037.3903438
GCAGAGT135800.034.5569049
GTACATG124200.032.8906361
TACATGG125500.032.279032
TAACGCA1501.483877E-731.7216874
ACATGGG125350.031.6489323
CATGGGG75950.031.0116564
ATGGGGG45550.028.8576835
AGAGTAC131900.028.04808210-11
AGTACTT67500.026.6550312-13
CATGGGT22250.026.4644414
TGGGGGG38600.026.3491366
Produced by FastQC (version 0.11.5)