FastQCFastQC Report
Fri 10 Feb 2017
ERR1147925.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147925.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1547896
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112350.7258239571650809No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68370.4416963413562668No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54000.3488606469685302No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19340.12494379467354395No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA85800.051.7642441
GGTATCA47750.046.506521
AACGCAG103000.042.795146
TCAACGC112800.039.3407974
ATCAACG113300.039.219693
CAACGCA114750.038.6722645
TATCAAC115300.038.544362
ACGCAGA120200.036.7703557
CGCAGAG122500.036.225658
GCAGAGT132650.033.58839
GTACATG112900.032.114541
TACATGG115700.031.3664992
ACATGGG115450.031.018163
CATGGGG65200.029.8341454
AGAGTAC127100.028.29203410-11
ATGGGGG31600.028.0486935
CATGGGT19400.027.5965144
GAGTACT67000.026.10273412-13
AGTACTT69650.026.04906712-13
CAGAGTA129350.025.96037510-11
Produced by FastQC (version 0.11.5)