FastQCFastQC Report
Fri 10 Feb 2017
ERR1147924.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147924.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2157313
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT219091.0155689044658796No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126890.588185395443313No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110360.5115622999536924No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38450.1782309752919488No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32650.15134567862892404No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC31740.14712746829041498No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA150550.052.168041
GGTATCA84850.046.596871
AACGCAG180050.043.2097476
TATCAAC198300.039.3930782
ATCAACG198250.039.2438623
TCAACGC199200.039.085664
CAACGCA202100.038.554245
ACGCAGA208300.037.4066777
CGCAGAG211450.036.7931678
GCAGAGT226000.034.503369
GTACATG192950.034.5014651
TACATGG198950.033.6124532
ACATGGG195200.033.4275133
ATGGGGG62500.032.6423385
CATGGGT34050.032.490994
CATGGGG115100.031.5225734
TGGGGGG49950.029.6504296
AGAGTAC221950.028.3931110-11
ATGGGTG23500.027.0821445
GAGTACT116750.026.517412-13
Produced by FastQC (version 0.11.5)