FastQCFastQC Report
Fri 10 Feb 2017
ERR1147923.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147923.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2259212
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT208800.9242160540931971No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120880.5350538152240693No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104710.4634801868970243No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34460.1525310595021627No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27830.12318454399144481No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA146650.051.6436731
GGTATCA82950.049.2402041
AACGCAG173400.043.7312936
TCAACGC191400.039.587564
ATCAACG191400.039.494343
CAACGCA195500.038.7877585
TATCAAC198000.038.333992
ACGCAGA200500.037.998467
CGCAGAG202000.037.7162938
GCAGAGT216950.035.1720969
CGTATAC1251.2830242E-633.3057563
GTACATG182950.033.097911
TACATGG185950.032.3410032
ACATGGG187100.031.5332783
AGAGTAC210950.029.05358110-11
CATGGGT42850.028.8697974
CATGGGG97150.028.7730774
GAGTACT108900.027.85309812-13
ATGGGGG41300.027.5051865
CAGAGTA213800.027.47012510-11
Produced by FastQC (version 0.11.5)