FastQCFastQC Report
Fri 10 Feb 2017
ERR1147922.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147922.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1034105
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT224912.174924209823954No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147611.4274179121075714No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121131.1713510716996822No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39460.38158600915767743No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24310.23508251096358684No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA17830.17241962856769863No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16780.16226592077206858No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15620.15104849120737257No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15160.14660020017309655No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA13040.12609938062382448No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA12990.12561587072879446No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12610.12194119552656645No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12070.11671928866024243No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA11680.11294791147900841No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10780.10424473336846837No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84950.050.3309061
GTATCAA146100.048.584721
AACGCAG170150.041.6690646
TATCAAC191050.037.1783182
ATCAACG192950.036.776053
TCAACGC195700.036.259274
ACGCAGA197750.036.0036967
CGCAGAG198150.035.8409658
CAACGCA200000.035.4499555
CGCGATT350.002789516533.999958110-111
GCAGAGT209100.033.992519
CATGGGT20200.033.56784
ATGGGTG15350.031.774225
GTACATG153400.030.4343741
TACATGG154600.030.3213922
ACATGGG154250.030.0387383
AGAGTAC207400.029.12330210-11
ATGGGAG24600.028.7727455
GGGGTGA21950.028.1817677
CATGGGG61100.027.7442554
Produced by FastQC (version 0.11.5)