FastQCFastQC Report
Fri 10 Feb 2017
ERR1147921.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147921.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1083356
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35750.3299930955290782No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31460.2903939240655888No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27910.25762537891514886No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13950.071.71681
GTATCAA26050.054.8642351
TCAACGC36000.039.4856874
CAACGCA36500.039.1077355
ATCAACG36800.038.62733
AACGCAG37350.038.0584956
TATCAAC41600.034.3211982
ACGCAGA43200.032.9047367
CGCAGAG45100.031.5185098
AGAGTAC44950.028.6465610-11
GCAGAGT50250.028.5249739
CGTACCC900.0095472126.4339313
AGTACTT26650.025.8883912-13
GAGTACT25000.025.57482712-13
CAGAGTA47800.025.50763710-11
GTACATG37400.025.4761711
ACATGGG38650.024.0059763
GTATAGG3750.023.8202231
TACATGG39500.023.796032
GTACTTT28250.023.05366314-15
Produced by FastQC (version 0.11.5)