FastQCFastQC Report
Fri 10 Feb 2017
ERR1147919.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147919.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4618410
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162860.3526321829374178No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93090.20156287553508676No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75340.16312973512529202No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA120400.059.502461
GGTATCA58900.058.2375341
TCAACGC154350.046.199194
CAACGCA156500.045.4884955
ATCAACG159300.044.688953
AACGCAG161300.044.2823266
TATCAAC176600.040.4594422
ACGCAGA186450.038.2134487
CGCAGAG194000.036.7262768
GCAGAGT217100.033.0650569
GTACATG196950.031.9001161
TACATGG206350.030.186292
ACATGGG204450.029.8166283
AGAGTAC199400.027.45493710-11
GAGTACT94650.025.542412-13
CAGAGTA209100.025.32804710-11
CATGGGG117000.025.1617644
CATGGGT51800.024.4552234
AGTACTT98900.024.38464212-13
GTACTTT103350.023.39224214-15
Produced by FastQC (version 0.11.5)