FastQCFastQC Report
Fri 10 Feb 2017
ERR1147918.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147918.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2871619
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225480.785201658019396No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131330.45733782928724176No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115770.40315236805439725No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34180.11902693219399928No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA147500.057.933831
GGTATCA76200.057.4387131
AACGCAG199250.042.566846
TATCAAC200350.042.549092
TCAACGC201500.042.239124
ATCAACG202900.041.9183543
CAACGCA206050.041.3063935
ACGCAGA231600.036.5440447
CGCAGAG235600.035.9740948
GCAGAGT255500.033.265319
GTACATG205250.030.8695851
TACATGG211050.029.9627292
ACATGGG208200.029.5957453
CATGGGG108900.027.9635524
AGAGTAC245250.027.96214910-11
AGTACTT131400.026.5700912-13
GAGTACT126600.026.52042612-13
CAGAGTA252150.025.55760810-11
CATGGGT32350.024.6365994
CATGGGA59900.023.731294
Produced by FastQC (version 0.11.5)