FastQCFastQC Report
Fri 10 Feb 2017
ERR1147917.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147917.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1861479
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88640.47618049948454966No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53130.28541820777994276No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46890.25189647586677044No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA64950.059.3102871
GGTATCA31700.059.1638151
TCAACGC87850.043.8715484
AACGCAG88750.043.1585856
ATCAACG89750.042.9427873
CAACGCA89900.042.8049775
TATCAAC93700.041.2059362
ACGCAGA102050.037.5338027
CGCAGAG106100.036.1010788
GCAGAGT114600.033.631029
GTACATG105650.032.2352751
TACATGG109700.031.0201642
ACATGGG109550.030.4579183
AGAGTAC107350.028.31186910-11
CATGGGG59400.027.7359474
CATGGGT21250.026.8696484
GAGTACT48350.026.07888812-13
CAGAGTA111650.025.57008710-11
AGTACTT52100.024.82968512-13
ATGGGGG26650.024.1032855
Produced by FastQC (version 0.11.5)